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Synthetic Peptides in HIV Antibody Screening and Typing a
Author(s) -
VARNIER OLIVIERO E.,
NÄRVÄNEN ALE,
KORKOLAINEN MIRJA,
LILLO FLAVIA,
KONTIO SARI,
ELM JOSEPH,
SUNI JUKKA,
VAHERI ANTTI,
HUHTALA MARJALIISA
Publication year - 1991
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1991.tb37942.x
Subject(s) - library science , medicine , human immunodeficiency virus (hiv) , family medicine , computer science
We have defined continuous native epitopes of HIV proteins by using a systematic epitope-scanning technology. We have demonstrated that there is a highly immunoreactive continuous native epitope region in the transmembrane protein gp41 of HIV-1 that is immunoreactive with all studied HIV-1 antibody-positive sera. The corresponding region in HIV-2 gp34 behaves similarly. There is a clear difference, however, between HIV type 1 and type 2 transmembrane proteins in the number of highly immunoreactive regions, when presented properly as synthetic antigens in solid-phase EIA, can provide tests unusually suitable for early and reliable diagnosis of HIV-1 and HIV-2 infections and for type-specific distinction of the two types of HIV infections.