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Effects of General Anesthetics and Pressure on Mammalian Excitatory Receptors Expressed in Xenopus Oocytes
Author(s) -
DANIELS S.,
ZHAO D. M.,
INMAN NANCY,
PRICE D. J.,
SHELTON C. J.,
SMITH E. B.
Publication year - 1991
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1991.tb33834.x
Subject(s) - kainate receptor , excitatory postsynaptic potential , xenopus , inhibitory postsynaptic potential , nmda receptor , chemistry , receptor , agonist , gabaa receptor , kainic acid , neuroscience , glutamate receptor , biophysics , biology , biochemistry , ampa receptor , gene
The effects of general anesthetics and pressure on receptors from the mammalian central nervous system have been investigated using oocyte expression techniques. Poly A+ mRNA extracted from rat whole brain was injected into mature Xenopus oocytes producing depolarizing responses to the fast excitatory neurotransmitters NMDA and kainate and the inhibitory neurotransmitters GABA and glycine. An apparatus was constructed to allow agonist dose-response curves to be determined at high pressures using voltage-clamped oocytes. This was used to investigate the excitatory transmitter kainate. It was found that anesthetics depress the current induced by kainate whereas pressure does not appear to affect the responses associated with this transmitter. Furthermore it was found that pressure does not reverse (or modify in any way) the changes in response brought about by application of anesthetics.