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Anti‐Idiotypic Immunity as a Potential Regulator in Myeloma and Related Diseases
Author(s) -
HOLM GÖRAN,
BERGENBRANT SUSANNE,
LEFVERT ANNKARI,
YI QING,
ÖSTERBORG ANDERS,
MELLSTEDT HÅKAN
Publication year - 1991
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1991.tb33449.x
Subject(s) - idiotopes , immunology , epitope , cd8 , idiotype , waldenstrom macroglobulinemia , multiple myeloma , antibody , macroglobulinemia , monoclonal , biology , monoclonal antibody , microbiology and biotechnology , medicine , immune system , lymphoma
In this paper some recent and partly preliminary results on anti-idiotypic immunity against clonal B cells in human monoclonal gammopathies are summarized. B cell lines producing antibodies to idiotypic determinants on autologous monoclonal immunoglobulin could be propagated after activation with Epstein-Barr virus of peripheral blood lymphocytes from patients with MGUS and MM clinical stage I but not from untreated persons with advanced MM. Blood T lymphocytes from patients with MGUS and Waldenström's macroglobulinemia were activated to DNA synthesis and production of interleukins by the autologous M protein. In another series of experiments T cell clones raised from patients with MM clinical stage I and MGUS bound F(ab')2 fragments of the autologous M protein and were stimulated to DNA synthesis by the idiotope-bearing protein. Control experiments demonstrated the specificity for idiotypic determinants. Ten of eleven clones were CD4-/CD8+. Finally, using a panel of 8 mAbs to alpha/beta V region epitopes, we noted a clonal expansion of CD4+ and CD8+ T cells in MGUS and MM patients.