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First Expression of Protamine Message in Trout Testis a
Author(s) -
SENIUK NADINE A.,
TATTON WILLIAM G.,
CAN PAUL D.,
GARBER ANTHONY T.,
DIXON GORDON H.
Publication year - 1991
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1991.tb27315.x
Subject(s) - queen (butterfly) , library science , research centre , gelsolin , gerontology , medicine , biology , ecology , genetics , actin , hymenoptera , computer science
In situ hybridizations were performed using a biotinylated riboprobe complementary to protamine messenger RNA in order to directly examine the various cell types in the trout testis for the presence of protamine message. Computer-aided optical density measurements were used to provide estimates of transcript abundance for cells identified by their DAPI-labeled nuclei. Optically detectable protamine hybridization occurred only in spermatid cells. These findings are in accord with results obtained in other species which report protamine mRNA only in the post-meiotic spermatid cell; but they are in conflict with a previous study employing solution hybridization which noted that protamine message first appears in the spermatocytes of rainbow trout.