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Transformation of a Methylotrophic Bacterium, Methylobacterium extorquens , with a Broad‐Host‐Range Plasmid by Electroporation
Author(s) -
UEDA SHUNSAKU,
MATSUMOTO SEIJI,
SHIMIZU SHOICHI,
YAMANE TSUNEO
Publication year - 1991
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1991.tb18568.x
Subject(s) - agriculture , library science , agricultural science , engineering , geography , environmental science , computer science , archaeology
Electroporation was used to transform the methylotrophic bacterium Methylobacterium extorquens with broad-host-range plasmid pLA2917, which contains a gene specifying resistance to kanamycin. Plasmid DNA was introduced into M. extorquens in the presence of an electric pulse, and kanamycin-resistant transformants were obtained. These transformants harbored plasmid DNA that was identical to plasmid pLA2917. We examined several factors independently and found up to 8 x 10(3) transformants per microgram of DNA using 10 pulses with a duration of 300 microseconds at a field strength of 10 kV/cm.