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Translocation Mechanism of Cardiac Na‐Ca Exchange a
Author(s) -
LI JINMING,
KIMURA JUNKO
Publication year - 1991
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1991.tb17288.x
Subject(s) - chemistry , chromosomal translocation , ion , ionic bonding , ion exchange , analytical chemistry (journal) , guinea pig , biophysics , medicine , chromatography , biochemistry , biology , organic chemistry , gene
In single cardiac ventricular cells of guinea pig, we have studied the ionic translocation mechanism of the electrogenic Na-Ca exchange, that is, whether Na and Ca ions countercross the membrane simultaneously or consecutively. The dose-response relations between the external Ca ([Ca]o) and the outward Na-Ca exchange current were measured at three different internal Na concentrations ([Na]i) in the absence of external Na. Hyperbolic regression curves and Hanes-Woolf linear plots of the dose-response relation revealed that apparent Km values for external Ca (K'mCao) decrease progressively as [Na]i decreases. The ratio of K'mCao to apparent Imax value (I'max) showed a slight increasing tendency as [Na]i decreased. We previously interpreted the data as consistent with the simultaneous mechanism but without statistical analysis. Here we performed careful statistical analysis, which indicated that the K'max/I'max values were not significantly different among the different [Na]i at most of the potentials. This result suggests that Na-Ca exchange is likely to be a consecutive mechanism.