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Role of Interleukin‐4 in the Negative Regulation of Proliferation of Chronic Myelomonocytic Leukemia Cells a
Author(s) -
HARADA MINE,
AKASHI KOICHI,
SHIBUYA TSUNEFUMI,
TAKAMATSU YASUSHI,
NIHO YOSHIYUKI
Publication year - 1991
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1991.tb17235.x
Subject(s) - chronic myelomonocytic leukemia , interleukin , leukemia , cancer research , immunology , chemistry , cytokine , medicine , bone marrow , myelodysplastic syndromes
We studied the effects of interleukin-4 (IL-4) on the spontaneous proliferation of chronic myelomonocytic leukemia (CMMoL) cells in vitro to clarify whether IL-4 can act as a negative regulator of hematopoiesis. The results clearly show that IL-4 suppressed spontaneous DNA synthesis and colony formation by CMMoL cells in vitro. Colony formation by CMMoL cells was substantially suppressed by adding either an anti-IL-6 or anti-GM-CSF antibody to the colony assay system. This suppression was abrogated by the treatment of IL-4 with an anti-IL-4 antibody. Furthermore, the production of IL-6 or GM-CSF was markedly inhibited by adding IL-4 to the culture system. These observations indicate that IL-4 can act as a negative regulator for the autocrine growth of CMMoL cells by inhibiting their production of IL-6 and/or GM-CSF.