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Biochemical Properties of a P 2Y ‐Purinergic Receptor
Author(s) -
HARDEN T. KENDALL,
BOYER JOSÉ LUIS,
BROWN H. ALEXANDER,
COOPER CHRISTY L.,
JEFFS ROGER A.,
MARTIN MICHAEL W.
Publication year - 1990
Publication title -
annals of the new york academy of sciences
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1990.tb37677.x
Subject(s) - purinergic receptor , receptor , p2y receptor , phospholipase c , chemistry , biochemistry , radioligand , purinergic signalling , g protein , membrane , biophysics , microbiology and biotechnology , biology , adenosine receptor , agonist
The turkey erythrocyte has substantial value as a model for the study of a receptor that exhibits pharmacological properties very similar to those delineated in mammalian tissues for a P2Y-purinergic receptor. The G protein-dependent coupling of this receptor to phospholipase C can be studied in detail, and the availability of an abundant source of homogeneous cells from which highly purified plasma membranes can be prepared, has led to the development of a radiolabeled, reversibly binding radioligand for a P2Y-purinergic receptor and a photoaffinity covalent radiolabel for this receptor. This source of plasma membranes highly enriched in P2Y-purinergic receptors should also serve as a rich starting material for the eventual purification and structural characterization of this important signaling protein.