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Bioreactor Development for Production of Viral Pesticides or Heterologous Proteins in Insect Cell Cultures a
Author(s) -
SHULER M. L.,
CHO T.,
WICKHAM T.,
OGONAH O.,
KOOL M.,
HAMMER D. A.,
GRANADOS R. R.,
WOOD H. A.
Publication year - 1990
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1990.tb24260.x
Subject(s) - library science , computer science
The insect cell-baculovirus expression system has significant potential for producing proteins requiring some degree of posttranslational modification. T. ni cells appear to be as good a host as S. frugiperda cells for heterologous protein production as demonstrated by production of beta-galactosidase. Attachment-dependent cells of T. ni can be effectively cultured in a packed-bed reactor using glass beads. When cell in such a reactor were infected, they produced 35% of the total protein as beta-galactosidase. No cell detachment was observed even 70 h postinfection. A model of viral entry has been proposed and tested.