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Stoichiometry and Kinetics of Xylose Fermentation by Pichia stipitis a
Author(s) -
SLININGER P. J.,
BRANSTRATOR J. E.,
LOMONT J. M.,
DIEN B. S.,
OKOS M. R.,
LADISCH M. R.,
BOTHAST R. J.
Publication year - 1990
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1990.tb24232.x
Subject(s) - agriculture , research center , geography , political science , archaeology , law
Conclusions of previous investigations have led us to focus on Pichia stipitis as a yeast with high potential for producing ethanol from xylose-rich, wood-processing wastes. l SGiven 150 giL xylose in complex medium, strain Y-7124 functions optimally at 25-26°C and pH 4-7 to accumulate 56 giL ethanol with negligible xylitol by-production. In a past report, we cited the need for an optimal bioreactor system; toward this end, we put oxygen uptake, growth, and death kinetics into mathematical form.) The present report builds on our previous work as the pathways and stoichiometry of xylose metabolism are examined and models of xylose uptake and ethanol production are identified. Yeasts begin xylose metabolism with the sequence, xylose -+ xylitol-+ xylulose-+ xylulose 5-phosphate. In balanced form, this sequence is: