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Anti‐HIV Effects of CD4‐ Pseudomonas Exotoxin on Human Lymphocyte and Monocyte/Macrophage Cell Lines
Author(s) -
ASHORN PER,
MOSS BERNARD,
BERGER EDWARD A.
Publication year - 1990
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1990.tb17835.x
Subject(s) - exotoxin , pseudomonas exotoxin , monocyte , microbiology and biotechnology , macrophage , glycoprotein , biology , toxin , virology , cell culture , recombinant dna , lymphocyte , immunology , in vitro , biochemistry , gene , genetics
CD4(178)-PE40 is a recombinant protein consisting of the HIV envelope glycoprotein-binding region of human CD4 linked to active domains of Pseudomonas aeruginosa exotoxin A. The hybrid toxin selectively kills HIV-infected human T-cell lines and protects against HIV spread in mixtures of uninfected and infected cells. We now report that CD4(178)-PE40 also selectively kills chronically HIV-1-infected cells of monocyte/macrophage lineage. The results provide further support for therapeutic use of this hybrid toxin in the treatment of HIV-infected individuals.