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Expression of Mouse Protamine 1 Genes in Transgenic Mice a
Author(s) -
PESCHON JACQUES J.,
BEHRINGER RICHARD R.,
PALMITER RICHARD D.,
BRINSTER RALPH L.
Publication year - 1989
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1989.tb25897.x
Subject(s) - annals , medical school , medicine , library science , gerontology , physiology , history , classics , medical education , computer science
Mouse protamine genes are expressed exclusively in spermatids. Mouse protamine 1 (mP1) transcriptional regulatory elements can target the expression of either marked mP1 transgenes or mP1 chimeric genes to spermatids in transgenic mice. Sequences between -40 and -465 bp relative to the transcription start site are required for expression in spermatids, whereas sequences 3' of the point of translation initiation are dispensable. mP1 transcriptional regulatory sequences were used to direct the expression of a toxic gene product to spermatids. The phenotypic consequences of toxin expression in spermatids are described.