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An Automated Perifusion System for the Study of Rat Spermatogenesis in Vitro a
Author(s) -
KIERSZENBAUM ABRAHAM L.,
TRES LAURA L.
Publication year - 1987
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1987.tb25005.x
Subject(s) - spermatogenesis , meiosis , sertoli cell , in vitro , biology , mitosis , microbiology and biotechnology , medicine , endocrinology , andrology , genetics , gene
We report details of an automated perifusion system for the study of spermatogenesis in vitro. A potential of this new system is the evaluation of hormone/growth factor effects on the mitotic clonal expansion of spermatogonia and the meiotic and postmeiotic clonal differentiation of spermatogenic cells in coculture with Sertoli cells and in incubated seminiferous tubular segments.