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Location of the Guanosine Triphosphate (GTP) Hydrolysis Site in Microtubules a
Author(s) -
CAPLOW MICHAEL
Publication year - 1986
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1986.tb38428.x
Subject(s) - gtp' , tubulin , microtubule , guanosine triphosphate , guanosine , protein subunit , chemistry , guanosine diphosphate , hydrolysis , gtpase , biophysics , microbiology and biotechnology , biochemistry , biology , enzyme , gene
The rate for GTP hydrolysis remains approximately constant during microtubule assembly from microtubular protein. This indicates that GTP hydrolysis does not accompany tubulin-GTP subunit addition to microtubule ends. We suggest that GTP, within tubulin-GTP subunits that are incorporated into microtubules, is hydrolyzed predominantly at one or both microtubule ends at an interface of a cap of tubulin-GTP subunits and a core of tubulin-GDP subunits.