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Molecular Genetic Analysis of AMP‐dependent Protein Kinase
Author(s) -
GOTTESMAN MICHAEL M.,
FLEISCHMANN ROBERT,
ABRAHAM IRENE
Publication year - 1986
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1986.tb15529.x
Subject(s) - mutant , gene , protein kinase a , biology , protein subunit , cloning (programming) , phenotype , microbiology and biotechnology , genetics , dna , transcription (linguistics) , kinase , linguistics , philosophy , computer science , programming language
Genetic evidence has been obtained indicating that the responsiveness of CHO cells to cAMP requires an intact cADepPK system. DNA carrying mutant RI and C subunit genes has been transferred to wild-type cells. Recipient cells carrying the DNA have been detected by selecting for expression of the phenotype for cAMP resistance. We are in the process of cloning a functional mutant RI subunit to use as a moveable genetic element that can confer cAMP resistance on recipient cells. This cloned gene should allow us to test hypotheses concerning the mechanism of cAMP regulation of transcription in mammalian cells.