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Monitoring the LH Surge by Ultrarapid and Highly Sensitive Immunofluorometric Assay
Author(s) -
STENMAN ULFHAKAN,
ALFTHAN HENRIK,
KOSKIMIES AARNE,
SEPPäLä MARKKU,
PETTERSSON KIM,
LÖVGREN TIMO
Publication year - 1985
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1985.tb37563.x
Subject(s) - obstetrics and gynaecology , medicine , gynecology , university hospital , library science , family medicine , pregnancy , biology , genetics , computer science
A novel time-resolved immunofluorometric method is described for the estimation of human luteinizing hormone (LH) in serum and urine. The method utilizes two monoclonal antibodies: one reacting with the beta-subunit is adsorbed to the wall of a microtiter well, and the other is labeled with a fluorescent europium chelate and reacts with the alpha-subunit. The method is ultrarapid (15-30 min) and highly sensitive (1 IU/L). A large linear measuring range allows measurement of LH levels from 1 to 250 IU/L. For the monitoring of urinary LH, IFMA gives the same information as the Hi-Gonavis assay, but it has the advantages of greater sensitivity and a shorter assay time. For the determination of serum LH levels, an acceptable correlation was observed between radioimmunoassay and IFMA. Furthermore, IFMA has a greater sensitivity, and is more rapid and not dependent on the handling of radioactive materials.