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Electrophysiological Studies of Sodium Cotransport in Epithelia: Toward a Cellular Model a
Author(s) -
SCHULTZ STANLEY G.,
HUDSON RANDALL L.,
LAPOINTE JEANYVES
Publication year - 1985
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1985.tb14857.x
Subject(s) - electrophysiology , cotransporter , sodium , chemistry , biophysics , isotopes of sodium , neuroscience , biology , organic chemistry
During the past two decades, microelectrophysiological studies of small intestine and renal proximal tubule employing conventional as well as ion-selective microelectrodes have contributed significantly to our understanding of the nature of Na-coupled entry processes at the apical membrane as well as the overall workings of the simple model illustrated in FIGURE 1. These studies have unequivocally established the rheogenic and conductive nature of the Na-coupled sugar and amino-acid entry processes across the apical membrane of small intestine (and renal proximal tubule) and have, in addition, disclosed that the properties of the basolateral membrane respond to an increase in Na-coupled solute entry with an increase in the ability of the Na-K pump to extrude Na with little or no change in (Na)c32 and a parallel increase in the conductance of that barrier to K. Although these responses may be "triggered" by cell swelling, it is unclear how a cell "recognizes" minimal swelling and how this recognition, in turn, culminates in the observed changes in basolateral membrane pump-leak properties. Clearly, these findings have brought us to the interfaces between cell physiology and cell and molecular biology and have raised a number of intriguing questions that focus on the more global question: How do epithelial cells work?