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Large‐scale Production of Mammalian Cells and Their Products: Engineering Principles and Barriers to Scale‐up
Author(s) -
GLACKEN M. W.,
FLEISCHAKER R. J.,
SINSKEY A. J.
Publication year - 1983
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1983.tb47912.x
Subject(s) - microcarrier , biochemical engineering , production (economics) , productivity , scale (ratio) , process engineering , bioreactor , process (computing) , cell culture , scale up , production cost , chemistry , computer science , microbiology and biotechnology , pulp and paper industry , environmental science , cell , biochemistry , biology , engineering , organic chemistry , mechanical engineering , physics , genetics , classical mechanics , quantum mechanics , economics , macroeconomics , operating system
Mammalian cell products have great medical and clinical importance, but to date, production methods employed to manufacture these products on a large scale are not as cost efficient as they could be. The implementation of process control would greatly improve the productivity of these products. Recently developed methods to produce cells on a large scale, such as microcarriers, artificial capillaries, tubular spiral film, and microencapsulation must be optimized, and the problem of oxygen transfer limitation must be solved. The accumulation of potentially toxic waste products can inhibit growth and reduce productivity. This effect can be reduced by either adjusting the environmental parameters of a fed-batch culture, so that the cell's metabolism is shifted away from producing these compounds, or by continually perfusing medium through the culture. If these technical barriers can be overcome, the cost of producing products derived from mammalian cells can be greatly reduced.