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THE VERSATILITY OF DROSOPHILA MELANOGASTER FOR MUTAGENICITY TESTING *
Author(s) -
Valencia Ruby
Publication year - 1983
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1983.tb47825.x
Subject(s) - drosophila melanogaster , mutagenesis , drosophila (subgenus) , computer science , computational biology , organism , biology , genetics , mutation , gene
I trust that I have accomplished my aim in this presentation, to demonstrate that Drosophila mutagenesis testing protocols are varied and are adaptable. The choices are many, at different levels. First, the route of administration can be chosen that is most appropriate for the chemical compound or to duplicate the human exposure route or to obtain data needed for some particular purpose, such as comparative mutagenesis. Second, the genetic test scheme or combination of schemes can be chosen to suit the purpose of the testing (screening, risk evaluation, or comparative mutagenesis). Third, by treating the appropriate fly development stage and managing the mating and brooding procedures, the germ cell stage can be sampled that is the critical one for the mutagenic effect of that compound and the correct one for the end point to be observed. Fourth, in screening, using the SLRL assay, the experiment size can be adjusted in accordance with the test sensitivity desired, the projected use of the data, and time and cost restrictions. In summary, Drosophila melanogaster has long been known as a most versatile organism for genetic studies of all kinds--and we now know that this versatility extends to the evaluation of chemical compounds for mutagenic effects. If it were only a mammal!