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Localization of LDL in Arteries: Improvements in Immunofluorescence Procedures a
Author(s) -
HOFF HENRY F.,
FELDMAN DAVID L.,
GERRITY ROSS G.
Publication year - 1983
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1983.tb22200.x
Subject(s) - section (typography) , library science , citation , foundation (evidence) , medicine , associate editor , gerontology , management , classics , history , computer science , archaeology , economics , operating system
We have described the development of procedures used to localize LDL in arteries with atherosclerotic lesions from humans and experimental animal models. We have first illustrated data obtained from earlier cryostat studies, described the procedures, given examples of results of LDL localization in sections of paraffin-embedded blocks, and finally outlined the procedures used and examples for LDL localization in epoxy-embedded blocks. Future improvements in resolution of LDL localization will probably require performing electron microscopy on ultrathin sections of epoxy-embedded arteries followed by immunocytochemistry.