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INSERTION OF NEW GENETIC INFORMATION INTO BONE MARROW CELLS OF MICE: COMPARISON OF TWO SELECTABLE GENES *
Author(s) -
Mercola Karen E.,
BarEli Menashe,
Stang Howard D.,
Slamon Dennis J.,
Cline Martin J.
Publication year - 1982
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1982.tb43434.x
Subject(s) - selectable marker , mutant , gene , biology , thymidine kinase , haematopoiesis , dihydrofolate reductase , transformation (genetics) , microbiology and biotechnology , bone marrow , insertion , genetics , stem cell , virus , immunology , herpes simplex virus
A system for insertion of new genetic information into mouse hematopoietic cells is described. Two selectable genes were examined: herpesvirus thymidine kinase and a mutant mouse dihydrofolate reductase. The DHFR system appears to be superior in terms of the frequency and stability of gene insertion and expression in hematopoietic tissues. About 70% of mice had indirect (karyotypic) evidence of gene insertion; of these, about 60% (three of five) had stable expression of the inserted mutant DHFR. In contrast, only 13% of mice demonstrated stable karyotypic transformation by HSVtk, and of those with stable transformation five of seven showed persistent viral gene sequences in hematopoietic tissues.