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MICROINJECTION OF CLONED SV40 DNA FRAGMENTS IN THE STUDY OF CELL PROLIFERATION
Author(s) -
Galanti Norbel,
Soprano Kenneth,
Jonak Gerald,
Baserga Renato
Publication year - 1982
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1982.tb43431.x
Subject(s) - medical school , library science , medicine , gerontology , medical education , computer science
DNA recombinant technology and the manual microinjection technique were used to study the base sequences in the SV40 early gene coding for cell DNA replication and nucleolar activation. Sequences critical for rRNA gene activation are located between nucleotides 3826 to 3506 (0.39-0.33 m.u.). Base sequences from nucleotide 4350 to 4190 (0.49-0.46 m.u.) are required for cellular DNA replication. Major T antigen determinant is coded by a sequence extending from nucleotide 4190 to 3880 (0.46-0.42 m.u.). Considering that an increase in cell size is regularly accompanied by an increase in the cellular amount of rRNA, nucleolar genes should be a target for growth-in-size signals. Therefore, the SV40 early gene presents a domain responsible for cell growth in size, and another separate but proximate region coding for cellular DNA replication.