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THE ELEVATED REQUIREMENT FOR METHIONINE BY TRANSFORMED RAT LIVER EPITHELIAL CELLS IN VITRO
Author(s) -
Poirier Lionel A.,
Wilson Mary J.
Publication year - 1980
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1980.tb29533.x
Subject(s) - methionine , homocysteine , in vitro , methyltransferase , cell culture , methionine synthase , cell growth , biochemistry , biology , chemistry , methylation , genetics , amino acid , gene
Growth constants appear to constitute a useful tool in comparing the behavior for transformed and untransformed cells in culture. The substitution of homocysteine for methionine in culture medium caused a greater depression of growth with 3/4 of the transformed epithelial and with 10/14 other cell lines tested compared to the growth depression seen in similar untransformed lines. The greater growth depression exhibited by transformed lines on methionine-deficient, homocysteine-supplemented medium could generally be attributed to: 1. Greater growth of transformed cells in complete medium, and 2. The lower levels of methyltransferase generally found in the transformed lines. Finally methyltransferase appears to be a major determinant of growth of both normal an transformed cells growing in a methionine-deficient, homocysteine-supplemented medium.