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Phospholipids Containing Photoactivable Groups in Studies of Biological Membranes *
Author(s) -
Radhakrishnan R.,
Gupta C. M.,
Erni B.,
Robson R. J.,
Curatolo W.,
Majumdar A.,
Ross A. H.,
Takagaki Y.,
Khorana H. G.
Publication year - 1980
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1980.tb22099.x
Subject(s) - chemistry , membrane , liposome , glycophorin , gramicidin , lipid bilayer , bilayer , phospholipid , biochemistry , stereochemistry
Photoactivable carbene precursors, aryl diazirines and trifluorodiazopropionates, were incorporated synthetically into the omega-positions of fatty acids, which were used to synthesize phospholipids. Extensive intermolecular C--H insertion reactions were demonstrated by photolysis of liposomes prepared from the above phospholipids. Structural analysis of the cross-linked products showed that the predominant sites of cross-linking were in the expected positions within the bilayer. Studies on the topography of a number of membrane proteins using the above phospholipids were initiated. Cross-linking of the photoactivable phospholipids to membrane-embedded proteins, glycophorin A, cytochrome b5, and gramicidin A, was demonstrated.