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DE NOVO CELL‐FREE SYNTHESIS OF HUMAN INTERFERON *
Author(s) -
Pestka Sidney,
Mcinnes James,
Weiss David,
Havell Edward A,
Vilček Jan
Publication year - 1977
Publication title -
annals of the new york academy of sciences
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1977.tb22005.x
Subject(s) - interferon , human cell , rna , cell , messenger rna , biology , protein biosynthesis , cell free system , chemistry , cell culture , microbiology and biotechnology , virology , biochemistry , gene , in vitro , genetics
Biologically active human interferon was synthesized de novo in a cell-free mouse extract stimulated with messenger RNA from induced human fibroblasts. The identity of the antiviral activity as human interferon was demonstrated by its antigenic and species specificity. Some characteristics of the cell-free synthesis were described.