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STUDIES ON DEFINED IMMUNOFLUORESCENCE IN CLINICAL IMMUNOPATHOLOGY. II. RELATIONSHIP OF F/P RATIOS OF CONJUGATES AND STAINING PROPERTIES IN INDIRECT IMMUNOFLUORESCENCE *
Author(s) -
Shu Suyu,
Deng JauShyong,
Vetter Susan,
Beutner Ernst H.
Publication year - 1975
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1975.tb29203.x
Subject(s) - immunofluorescence , staining , indirect immunofluorescence , conjugate , immunopathology , chemistry , microbiology and biotechnology , antibody , pathology , medicine , biology , immunology , mathematics , mathematical analysis
The effect of conjugate F/P ratios on titers of patient antibodies was evaluated with two model systems in IIF chessboard titrations, notably, antinuclear antibodies and the intercellular antibodies of pemphigus. The plateau titers of both antibodies and F/P conjugate ratios were analyzed and proved to have a high degree of linear relationship statistically. Single titrations of other tissue antibodies, including skin basement membrane antibodies of bullous pemphigoid, MTA, GPA, SMA, and ThA with the same group of conjugates yielded similar information. A quantitative relationship between the two parameters was established.