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SYNTHESIS OF α‐FETOPROTEIN BY RAT ASCITES HEPATOMA CELLS
Author(s) -
Kanai Koichi,
Endo Yasuo,
Oda Toshitsugu,
Kaneko Yoshiyasu
Publication year - 1975
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1975.tb25399.x
Subject(s) - medicine , library science , computer science
AH-66 rat ascites hepatoma cells incorporated [14C]leucine into the AFP fraction. In a cell-free system, hepatoma ribosomes were found to be active in AFP synthesis whereas the supernatant fraction from hepatoma had no specific effect on AFP production. The amount of [14C]leucine incorporated in AFP by membrane-bound polysomes was 20 to 90 times higher than that by free polysomes, suggesting that AFP is mainly synthesized on membrane-bound polysomes. DBcAMP inhibited the growth of hepatoma cells. However, the incorporation of [14C]leucine into the AFP fraction as well as into total proteins was stimulated by DBcAMP.