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FACTORS AFFECTING HEMOSTATIC PLUG FORMATION IN AN EXTRACORPOREAL MODEL *
Author(s) -
Didisheim Paul,
Pavlovsky Miguel,
Kobayashi Itsuro
Publication year - 1972
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1972.tb16307.x
Subject(s) - bleeding time , hemostasis , medicine , anesthesia , prothrombin time , coagulation , blood pressure , epinephrine , heparin , surgery , platelet aggregation , platelet
SUMMARY A model is described for the study of factors affecting hemostasis. It consists of a Teflon extension of the carotid artery of rats. Holes of precise diameter are then produced in the extension with a micromanipulator and the aid of a microscope. The bleeding time of these holes is then recorded. The principal advantage of this method is that it makes possible the production in the “blood vessel” of holes the diameters of which are known with a high degree of accuracy, under known conditions of intravascular pressure. With holes below 75 μ in diameter, prolongation of the coagulation time with heparin did not affect the bleeding time. With increasing hole diameters, or with increasing intravascular pressure, coagulation time did affect the bleeding time. Bleeding time could be predictably modified by increasing or decreasing blood pressure. There is a striking interrelationship of these factors in influencing the bleeding time. Intravenous pyridinolcarbamate (12 mg/kg) significantly prolonged the bleeding time for a 20‐minute period. At a lower dose (8 mg/kg), which was ineffective in prolonging the bleeding time, pyridinolcarbamate partially inhibited the shortening of the bleeding time that follows the administration of epinephrine. Intravenous polyvinylpyrrolidone (PVP), 750 mg/kg, caused a sustained prolongation of the bleeding time, lasting more than three hours.