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METHYL‐VITAMIN B 12 AS A SOURCE OF METHYL GROUPS FOR THE SYNTHESIS OF ACETATE BY CELL‐FREE EXTRACTS OF CLOSTRIDIUM THERMOACETICUM
Author(s) -
Poston J. Michael,
Kuratomi K.,
Stadtman E. R.
Publication year - 1964
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1964.tb45057.x
Subject(s) - library science , national library , medicine , chemistry , computer science
Glucose fermentation by Clostridium themnoaceticum leads to the accumulation of nearly three moles of acetate per mole of substrate dec0mposed.l The possibility that one of the three moles of acetate is derived indirectly from carbon dioxide was suggested by the discovery of Barker and Kamen2 that acetate, labeled almost equally in both carbon atoms, is produced when glucose is fermented in the presence of added CI4O2. This possibility was later confirmed by Wood's demonstration3 that nearly one-third of the acetate molecules produced during the dissimilation of glucose in the presence of C1302 is formed by a mechanism in which adjacent carbon atoms are derived from C1302. Thus, glucose fermentation by Cl. thermoaceticum is represented by the following overall reactions: CGH12O6 f 2H20 -+ ZCHBCOOH + 2CO2 + 8H ( 1 ) 8H + 2C02 + CH3COOH + 2H20 (2)