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ENZYMATIC SYNTHESIS OF DESOXYRIBONUCLEIC ACID *
Author(s) -
Lehman I. R.
Publication year - 1959
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.1959.tb49356.x
Subject(s) - citation , library science , chemistry , computer science
Our studies during the past 3 years on the synthesis of desoxyribonucleic acid (DNA) by an enzyme that we have partially purified from Escherichia coli have revealed the following basic features of this reaction. (1) The desoxynucleoside triphosphatest of the 4 predominant bases found in DNA-adenine, guanine, thymine, and cytosine-must be present for appreciable synthesis to occur. (2) Polymerized DNA and Mg++ are essential for the reaction. (3) Inorganic pyrophosphate is released in amounts equal to the amount of