Premium
Altered expressions of trefoil peptides genes in squamous cell carcinoma of the larynx
Author(s) -
Sipaul F.,
Corfield A.,
Wood S.,
Upile T.,
Sheffield E.,
Birchall M.A.
Publication year - 2008
Publication title -
clinical otolaryngology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.914
H-Index - 68
eISSN - 1749-4486
pISSN - 1749-4478
DOI - 10.1111/j.1749-4486.2008.01843_12.x
Subject(s) - mucin , medicine , carcinogenesis , larynx , laryngectomy , trefoil , pathology , immunohistochemistry , gene , carcinoma , cancer , laryngeal neoplasm , biology , anatomy , genetics , agronomy
Aim. Trefoil peptides (TFF) are secretory products of mucin‐producing cells and influence the rheological properties of mucous gels. 1 They are thought to be important for the repair of mucosa damage. 2 We hypothesised that TFF genes would be expressed in laryngeal mucosa and that this would be altered by smoking and neoplasia. Methods. The expressions of TFF1‐3 were examined using 35 S‐dATP in situ hybridisation. Biopsies were obtained from the supraglottic mucosa of non‐smokers (normal, n = 14) and smokers (smokers, n = 14) without laryngeal disease undergoing routine surgery and compared with tissue from archival squamous cell carcinoma (SCC: laryngectomy) specimens ( n = 14). Autoradiographic intensity was scored (semi‐quantitatively) by two independent observers and compared to positive and negative control tissue. Results. Scores are medians for each gene.TFF gene Normal ( n = 14) Smokers ( n = 14) SCC ( n = 14)TFF1 0 0 + TFF2 0/+ 0 + TFF3 ++ + ++Expression was scored as 0–+++; 0 = absent, +++ strongly positive.Discussion. Major expression of TFF3 and weak expression of TFF2 are found in normal laryngeal mucosa. TFF3 appears to be down‐regulated in smokers and then up‐regulated again in the cancer group (SCC). The significant findings are that TFF1 and 2 appear to be expressed or up‐regulated in the cancer group. These findings suggest that laryngeal squamous cell carcinogenesis is associated with abnormal TFF1 and TFF2 genes expressions. References. 1 Kutta H., Steven P., Varoga D. et al . (2004) TFF peptides in the human false vocal folds of the larynx. Peptides. 25(5), 811–818 2 Taupin D. & Podolsky D.K. (2003) Trefoil factors: initiators of mucosal healing. Nat Rev Mol Cell Biol . 4(9), 721–732