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Gamma tubulin: a powerful indicator of recurrence in human squamous cell carcinoma of the larynx
Author(s) -
Syed M.I.,
Syed S.,
Harrower S.,
Singh J.,
Chin A.,
McLellan D.,
Parkinson E.K.,
Clark L.J.
Publication year - 2007
Publication title -
clinical otolaryngology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.914
H-Index - 68
eISSN - 1749-4486
pISSN - 1749-4478
DOI - 10.1111/j.1749-4486.2007.01568_9.x
Subject(s) - medicine , immunostaining , pathology , cancer , larynx , antigen , staining , carcinoma , stage (stratigraphy) , cancer research , immunohistochemistry , biology , immunology , anatomy , paleontology
Objectives. Centrosome amplification as detected by gamma tubulin (GT) immunostaining is associated with genetic instability and tumour aggressiveness. 1 We have assessed GT for its ability to predict recurrence of squamous cell carcinoma of the larynx (SCCL). Methods. Five‐micrometer sections of 35 archival SCCL samples were subjected to antigen retrieval and immunostained with an antibody to GT. The keratin antibody (CK5) served as a positive control for antigen retrieval, and tonsillar tissue was used as a negative control. Results. Of the 35 tumours analysed, 22 suffered recurrence (R) and 13 did not (NR). 14 of the 22 R tumours, but 0 of 13 of the NR tumours had a GT staining score of 2+ or 3+ ( P = 0.0002 by the Fisher's exact Test). Recurrence was unrelated to T stage ( P = 0.726) and had a weak relationship to N status ( P = 0.211). Conclusion. GT staining appears to be a better indicator of tumour recurrence than either T stage or node status. Reference 1 Carroll P.E., Okuda M., Horn H.F. et al . (1999) Centrosome hyperamplification in human cancer: chromosome instability induced by p53 mutation and/or Mdm2 overexpression. Oncogene, 18, 1935–44