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Confirmed expression of MHC class I and class II genes in the New Zealand endemic Hector's dolphin ( Cephalorhynchus hectori )
Author(s) -
Heimeier Dorothea,
Baker C. Scott,
Russell Kirsty,
Duignan Pádraig J.,
Hutt Alistair,
Stone Gregory S.
Publication year - 2009
Publication title -
marine mammal science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.723
H-Index - 78
eISSN - 1748-7692
pISSN - 0824-0469
DOI - 10.1111/j.1748-7692.2008.00244.x
Subject(s) - biology , genetics , gene duplication , locus (genetics) , major histocompatibility complex , complementary dna , gene , nonsynonymous substitution , exon , genomic dna , mhc class ii , mhc class i , genome
We provide the first description and confirmation of expression for three Major Histocompatibility Complex (MHC) genes (DQA, DQB, and class I) in the New Zealand endemic Hector's dolphin ( Cephalorhynchus hectori ). Sequences were derived from cDNA generated from peripheral blood leukocyte mRNA collected during a temporary live capture of two Hector's dolphins. The complete exons 2 and 3 of DQA, DQB, and class I were amplified from cDNA to confirm their expression. The same primers proved reliable for amplification from genomic DNA. Locus identity was supported by phylogenetic relationships to MHC molecules from other closely related species. No evidence was found for duplication of DQ loci, but cloning of amplified exon 2 of the class I gene indicated duplication or triplication of this locus. However, the expression of multiple class I loci was uncertain as only one allele could be detected from clones of cDNA. Balancing selection was indicated by a significantly greater ratio of nonsynonymous to synonymous substitutions at all three loci and by the apparent transspecific sharing of alleles for DQA and DQB.