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ISOLATION AND CHARACTERIZATION OF A cDNA ENCODING INTERLEUKIN 2 FROM THE FLORIDA MANATEE, TRICHECHUS MANATUS LATIROSTRIS
Author(s) -
Cashman Mary E.,
Ness Traci L.,
Roess William B.,
Bradley W. Guy,
Reynolds John E.
Publication year - 1996
Publication title -
marine mammal science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.723
H-Index - 78
eISSN - 1748-7692
pISSN - 0824-0469
DOI - 10.1111/j.1748-7692.1996.tb00306.x
Subject(s) - complementary dna , manatee , biology , microbiology and biotechnology , polymerase chain reaction , zoology , gene , genetics , ecology
A bstract Interleukin‐2 (IL‐2) production is essential for optimal mammalian immune response; therefore, a cDNA encoding IL‐2 was isolated and characterized for the endangered Florida manatee, Trichechus manatus latirostris . The manatee IL‐2 cDNA was cloned by reverse transcription/polymerase chain reaction (PCR), using primers derived from IL‐2 regions conserved among various mammalian species. Manatee IL‐2 cDNA consisted of 450 base pairs encoding a predicted product of 150 amino acid residues and displayed 87%, 82%, 79%, 78%, 75%, and 73% DNA sequence homology and 74%, 69%, 63%, 62%, 61%, and 57% amino acid sequence homology with human, pig, sheep, cow, rat, and mouse IL‐2, respectively.