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CHARACTERIZATION OF A BOTTLENOSE DOLPHIN ( TURSIOPS TRUNCATUS ) KIDNEY EPITHELIAL CELL LINE
Author(s) -
Carvan Michael J.,
SANTOSTEFANO MICHAEL,
SAFE STEPHEN,
BUSBEE DAVID
Publication year - 1994
Publication title -
marine mammal science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.723
H-Index - 78
eISSN - 1748-7692
pISSN - 0824-0469
DOI - 10.1111/j.1748-7692.1994.tb00389.x
Subject(s) - biology , bottlenose dolphin , population , cyclin dependent kinase , cell culture , karyotype , hematoporphyrin , plating efficiency , microbiology and biotechnology , zoology , cell , cell cycle , genetics , ecology , chemistry , chromosome , demography , photodynamic therapy , organic chemistry , sociology , gene
An epithelial cell line, Carvan dolphin kidney (CDK), isolated from a prematurely born female bottlenose dolphin, Tursiops truncatus , exhibited growth characteristics not previously reported for cetacean cells in culture. CDK cells were cytokeratin positive and demonstrated a maximum doubling time of 1.31 days, with plating and colony forming efficiencies approaching 100% for the early population doublings. Despite an unusually efficient colony‐forming ability and rapid growth, these cells were neither transformed nor immortal, displaying normal contact inhibition, anchorage dependence, and the requirement for high concentrations of fetal bovine serum in the growth medium. CDK cells exhibited age‐dependent changes in growth rate, colony‐forming efficiency, and cytoplasmic profile, and showed a finite lifespan of about 50 population doublings and a stable 2N = 44 karyotype which correlates with previously reported cytogenetic analyses. Velocity sedimentation analysis showed that CDK cells contained nuclear aryl hydrocarbon receptor (Ah), indicating their potential to be induced for cytochrome P450. These data suggest that CDK cells may have utility as an in vitro toxicological model for evaluating hydrocarbon contaminant effects on Tursiops truncatus , a protected marine mammal.