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CROSS‐REACTIVITY OF ANTIBODIES TO HUMAN ANTIGENS WITH TISSUES OF THE BOTTLENOSE DOLPHIN, TURSIOPS TRUNCATUS, USING IMMUNOPEROXIDASE TECHNIQUES
Author(s) -
Kumar Dhruv,
Cowan Daniel F.
Publication year - 1994
Publication title -
marine mammal science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.723
H-Index - 78
eISSN - 1748-7692
pISSN - 0824-0469
DOI - 10.1111/j.1748-7692.1994.tb00260.x
Subject(s) - polyclonal antibodies , antigen , bottlenose dolphin , immunoperoxidase , antibody , cross reactivity , monoclonal antibody , biology , microbiology and biotechnology , immunohistochemistry , immunology , virology , cross reactions , ecology
A bstract Cross‐species reactivity of 53 antibodies developed against human antigens was evaluated in the bottlenose dolphin Tursiops truncatus , using immunoperoxidase techniques, Strong cross‐reactivity was demonstrated with antibodies against intermediate filaments, most hormones, but few leukocyte antigens. S100, NSE, F8RA, Factor XIIIa, and lactalbumin antibodies reacted, while HMB45, EMA, PLAP, and PSA did not. Polyclonal antibodies are much more likely to cross‐react than are monoclonal antibodies. Twenty‐four of 30 (80%) of polyclonals cross‐reacted, while only 10 of 23 (43.5%) monoclonals cross‐reacted. Some antigens are demonstrated only after using a special technique to unmask the antigen.