Premium
Evidence for FOXO transcription factor in the silkworm, Bombyx mori (Lepidoptera : Bombycidae)
Author(s) -
Kim Jin Hee,
Lee Bong Hee
Publication year - 2011
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2011.00424.x
Subject(s) - bombyx mori , foxo1 , biology , transcription factor , bombycidae , microbiology and biotechnology , insulin receptor , forkhead transcription factors , signal transduction , foxo3 , protein kinase b , insulin , apoptosis , starvation , insulin resistance , endocrinology , genetics , gene
Foxo transcription factors, including Foxo1, Foxo3a and Foxo4, are implicated in the regulation of several biological processes, including the stress resistance, metabolism and apoptosis. We have analyzed Foxo1 and Foxo3a, its regulation by insulin signaling pathway and apoptosis after starvation in the Bombyx mori fat bod y. Under starvation conditions, Foxo1 and Foxo3a are localized to the nucleus. Levels of the insulin receptor (InR), p‐AKT and p‐JNK gradually increased in the nucleus after starvation. Also, caspase‐3 and caspase‐mediated PARP were observed in early stages of starvation. In conclusion, it has been shown that Foxo transcription factors are localized to the nucleus by cellular stress and the state of starvation led to changes in insulin signaling pathway and apoptosis in B. mori. These data suggest that the Foxo transcription factors act downstream of insulin signaling pathway to regulate physiological process in B.mori. Our future studies will focus on the molecular mechanisms of signaling pathway in B.mori.