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Cellular and humoral immune defenses of Oxya japonica (Orthoptera: Acrididae) to entomopathogenic fungi Metarhizium anisopliae
Author(s) -
ANGGRAENI Tjandra,
PUTRA Ramadhani Eka
Publication year - 2011
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2010.00311.x
Subject(s) - metarhizium anisopliae , biology , acrididae , conidium , entomopathogenic fungus , grasshopper , orthoptera , metarhizium , acridoidea , microbiology and biotechnology , fungus , virulence , immune system , biological pest control , botany , beauveria bassiana , zoology , immunology , ecology , biochemistry , gene
One Indonesian isolate of the fungus Metarhizium anisopliae , named Majalengka strain, was evaluated not only for its virulence but also for the immune response of rice grasshopper Oxya japonica (Orthoptera: Acrididae) as a target organism. Five aqueous suspensions with different conidia concentrations in logarithmic series were prepared. The fungus showed high virulence as it caused 100% mortality at low conidia concentration (1.5 × 10 2 conidia/mL). Remarkable changes in the cellular and humoral responses were also observed when adult grasshoppers were infected with the fungus. The number of hemocytes decreased significantly within 12 h after infection. In addition, the total number of granulocytes increased rapidly in the first 12 h then gradually decreased 24 and 48 h after infections, while the number of coagulocytes fluctuated over time. The infection influenced the humoral response by increasing the phenoloxidase activity.