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Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella
Author(s) -
KIM Rye Jin,
OH Seung Han,
NOH Mi Young,
JO Yong Hun,
KIM Seongon,
BANG In Seok,
LEE Yong Seok,
KIM Iksoo,
HAN Yeon Soo
Publication year - 2010
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2010.00291.x
Subject(s) - diamondback moth , plutella , biology , calreticulin , open reading frame , anopheles gambiae , bombyx mori , complementary dna , innate immune system , nasonia vitripennis , microbiology and biotechnology , genetics , gene , peptide sequence , botany , endoplasmic reticulum , pteromalidae , immunology , host (biology) , immune system , parasitoid , lepidoptera genitalia , malaria
Calreticulin (CRT) plays pivotal roles in Ca 2+ homeostasis, molecular chaperoning, infection, inflammation and innate immunity. In an attempt to study the involvement of CRT in innate immunity, the full‐length cDNA of calreticulin ( PxCRT ) was cloned from the diamondback moth, Plutella xylostella . It consists of 1674 bp (excluding poly‐A tail) with a longest open reading frame (ORF) of 1197 bp encoding 398 amino acids. In silico analysis of PxCRT ORF reveals that it has various repeat motifs and endoplasmic reticulum retention signal found in all the calreticulin proteins. As expected, high amino acid sequence identities were found from other CRTs identified from Bombyx mori (87%), Galleria mellonella (87%), Apis mellifera (74%), Anopheles gambiae (74%), Tribolium castaneum (73%), Culex quinquefasciatus (73%), Rhodnius prolixus (72%), Nasonia vitripennis (71%), Drosophila melanogaster (71%) and Haemaphysalis qinghaiensis (68%). During development, P. xylostella expressed PxCRT predominantly in the pupal stage. In addition, spatial expression pattern analysis indicates that PxCRT was highly expressed in the silk gland. PxCRT mRNA, furthermore, was strongly induced 3 to 6 h after laminarin treatment, suggesting that PxCRT appears to be involved in immune responses and also plays an important role in the silk gland.