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Cloning and expression pattern of 14‐3‐3ε from Culex pipiens
Author(s) -
KIM Dong Hyun,
JO Yong Hun,
HAN Yeon Soo,
KANG Sang Sun,
CHUN Jaesun
Publication year - 2009
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2009.00223.x
Subject(s) - biology , anopheles gambiae , microbiology and biotechnology , malpighian tubule system , bombyx mori , messenger rna , midgut , drosophila melanogaster , culex pipiens , signal transduction , gene , genetics , immunology , larva , botany , malaria
14‐3‐3 proteins play various roles in eukaryotes, such as signal transduction, cell cycle regulation, apoptosis, stress response and cytoskeleton organization. We are interested in apoptosis induced by mosquito–virus interactions using Culex pipiens as an experimental model. To this end, we cloned a partial fragment (702 bp) of 14‐3‐3ε, one of the key proteins known to be involved in apoptosis, from Cx. pipiens . The deduced amino acid sequence (234 residues) had high homology to 14‐3‐3ε homologs from various organisms, such as Culex quinquefasciatus (99%), Anopheles gambiae (98%), Aedes aegypti (96%), Drosophila melanogaster (93%), Apis mellifera (91%) and Bombyx mori (89%). Developmental reverse transcription (RT)‐polymerase chain reaction (PCR) analysis showed that Cp14‐3‐3ε mRNA is expressed at very high levels in the egg, early pupa, and adult male and female, although it was expressed in other developmental stages, such as first and fourth instar larvae. Analysis of RT‐PCR for tissue specificity showed that Cp14‐3‐3ε is abundant in the abdomen and ovary, and it was also detectable in all other tissues, such as thorax, midgut and Malpighian tubule. Actinomycin‐D, an apoptosis‐inducing chemical, induces Cp14‐3‐3ε mRNA expression. Furthermore, poly I : C (RNA virus analog) induces Cp14‐3‐3ε mRNA 3 h after injection. Thus, although further investigation is necessary for elucidating the biological significance of the induced 14‐3‐3ε, our data suggest that Cp14‐3‐3ε is involved in cell survival and signal transduction in Cx. pipiens .