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Establishment of a Self‐Mating Mosquito Colony of Anopheles sinensis From Korea
Author(s) -
KIM SeJoo,
CHOOCHOTE Wej,
JITPAKDI Atchariya,
JUNKUM Anuluck,
PARK SooJoon,
MIN GiSik
Publication year - 2003
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2003.tb00080.x
Subject(s) - biology , anopheles sinensis , mating , vector (molecular biology) , zoology , first generation , strain (injury) , malaria , anopheles , ecology , veterinary medicine , genetics , demography , population , immunology , recombinant dna , anatomy , gene , medicine , sociology
Many Korean entomologists have attempted to colonize Anopheles sinensis Korean strain, a primary malaria vector in Korea, previous efforts, however, were unsuccessful. Only a study that a successful colonization of An. sinensis Korean strain over 5 generations was recently reported. Recently we obtained a self‐mating colony from this mosquito strain. The original colony was established from progenies obtained from females collected from So‐Rae District, Incheon, Republic of Korea (ROK), and was maintained for 23 successive generations. While the adult females of first five generations were fed on humans as a source of blood, those of the subsequent 18 generations were fed on white rats as a source of blood. A self‐mating colony was initiated from the 9th generation by rearing 200 and 300 newly emerged females and males, respectively, in a 30x30x30 cm cage. The insemination rates increased from approximately 40% to 81% by the 20 th generation. Comparisons of biological aspects between artificial mating and self‐mating colonies are reported.