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Lysozyme as Pathogen‐Recognition Protein in the Hemolymph of Galleria mellonella
Author(s) -
LEE In Hee
Publication year - 2003
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2003.tb00063.x
Subject(s) - hemolymph , lysozyme , galleria mellonella , biology , microbiology and biotechnology , antiserum , candida albicans , pathogen , immune system , biochemistry , antibody , virulence , immunology , gene
Recognition of invading micro‐organisms into hemolymph is a pivotal event for triggering diverse immune mechanisms in insects. It has been known that this recognition was mediated by the binding of hemolymph proteins to pattern‐molecules on the cell surface of microbes. Recently, I found that the lysozyme in the G. mellonella hemolymph has binding affinity to cell‐walls of Gram (‐), (±) bacteria and fungus ( Candida albicans ). After the hemolymph was incubated with heat‐killed microbes and treated with acidic buffer containing high concentration of NaCl, several plasma proteins detached from microbes were detected by reverse phase HPLC and SDS‐PAGE analyses. Of binding proteins, it was assumed that the major one might be a lysozyme, which was previously characterized in the G. mellonella hemolymph. Furthermore immunoblot analysis performed with antiserum to G. mellonella lysozyme revealed that it was a lysozyme.