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Characterization of High Density Lipophorin (HDLP) Receptor from the Fat Body of Galleria mellonella by Ligand Blot Analysis
Author(s) -
LEE ChangSeok,
HAN JikHyon,
KIM HakRyul
Publication year - 2002
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2002.tb00006.x
Subject(s) - galleria mellonella , suramin , biology , receptor , blot , ligand (biochemistry) , western blot , instar , molecular mass , chelation , microbiology and biotechnology , biochemistry , larva , chemistry , enzyme , ecology , organic chemistry , virulence , gene
To identify and characterize the HDLP (high density lipophorin) receptor from Galleria mellonella (LPR Gm ), we used techniques of ligand blotting. This method was, to our knowledge, firstly used to characterize the lipophorin receptor (LPR) in insect. LPR gm had the approximate molecular weight of 100 kDa under non‐reducing conditions and bound the HDLP very rapidly and specifically. The receptor had an absolute requirement of Ca 2+ but exceeding concentration of Ca 2+ has shown some inhibitory effect, and this result was supported by the effect of EDTA, here used for Ca 2+ chelating reagent. LPR Gm has the high binding affinity to HDLP and its binding with HDLP was rapid and specific. Suramin (polysulfated polycyclic hydrocarbon) effectively inhibited the LPR Gm and HDLP binding. LPR Gm showed the stage specific binding activity especially in day 1‐3 last instar larval, prepupal, and adult stages.