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Construction and Characterization of Transformed Insect Cells That Do Not Require Antibacterial Protein in Cell Culture System
Author(s) -
PARK Hye Jin,
LEE Kwang Sik,
CHO Eun Sook,
YUN Eun Young,
KANG Seok Woo,
KIM Keun Young,
HWANG Jae Sam,
SOHN Hung Dae,
JIN Byung Rae
Publication year - 2002
Publication title -
entomological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 20
eISSN - 1748-5967
pISSN - 1738-2297
DOI - 10.1111/j.1748-5967.2002.tb00001.x
Subject(s) - autographa californica , biology , nuclear polyhedrosis virus , cell culture , antibacterial activity , escherichia coli , microbiology and biotechnology , gene , bacteria , biochemistry , genetics , recombinant dna , spodoptera
Transformed Sf 9 cell line expressing antibacterial protein was constructed by using Autographa californica nuclear polyhedrosis virus (AcNPV) immediate early gene (iel). The cell line was resistant to neomycin and expressed antibacterial protein under the control of Drosophila heat shock protein promoter. The genomic organization and expression at the transcriptional level of nuecin in the transformed cells were characterized. Nuecin was successfully expressed in the transformed cells and secreted into the media. Antibacterial activity of nuecin from the transformed cells was assayed against Escherichia coli. It indicates that nuecin secreted from the transformed cells is possible, at least in permissible concentration, to recover the cultures that have been contaminated.