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Initial evaluation of canine urinary cystatin C as a marker of renal tubular function
Author(s) -
Monti P.,
Benchekroun G.,
Berlato D.,
Archer J.
Publication year - 2012
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/j.1748-5827.2012.01198.x
Subject(s) - cystatin c , medicine , creatinine , cystatin , renal function , urinary system , urology , urine , clinical significance , gastroenterology
O bjectives : To evaluate the performance of a particle‐enhanced turbidimetric assay for measuring canine urinary cystatin C and to investigate if the urinary cystatin C to creatinine ratio is higher in dogs with renal disease than in non‐renal disease dogs. M ethods : Urinary cystatin C was measured by particle‐enhanced turbidimetric assay using an avian antihuman cystatin C antibody and the performance of this assay was evaluated. Clinical relevance was tested in 46 dogs that were divided into three groups: healthy dogs (n=14), non‐renal disease dogs (n=17) and dogs with renal disease (n=15). R esults : The assay was linear (R2=0·99) and precise (mean intra‐ and inter‐assay coefficients of variation were 2·3 and 2·9%, respectively). The recovery was 111·5% and the limit of blank was 0·02 mg/L. Urinary cystatin C and urinary cystatin C to creatinine ratio differed significantly (P<0·001) between the three cohorts of dogs. C linical S ignificance : Measurement of cystatin C by particle‐enhanced turbidimetric assay performed with high precision and linearity. This assay can be processed on automated clinical chemistry analysers making it widely available to commercial laboratories. Urinary cystatin C to creatinine ratio can differentiate dogs with renal disease from dogs without renal disease. These preliminary results suggest that urinary cystatin C to creatinine ratio is a promising marker for evaluating renal tubular function.