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Haemoplasma infection is not a common cause of canine immune‐mediated haemolytic anaemia in the UK
Author(s) -
Warman S. M.,
Helps C. R.,
Barker E. N.,
Day S.,
Sturgess K.,
Day M. J.,
Tasker S.
Publication year - 2010
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/j.1748-5827.2010.00987.x
Subject(s) - medicine , clinical significance , polymerase chain reaction , mycoplasma , immune system , haemolysis , immunology , microbiology and biotechnology , biology , gene , biochemistry
O bjective : The aim of this study was to investigate whether the two canine haemoplasma species, Mycoplasma haemocanis and “ Candidatus Mycoplasma haematoparvum ,” are commonly associated with immune‐mediated haemolytic anaemia (IMHA) in UK dogs.M ethods : Three groups of dogs were recruited to the study: anaemic dogs with primary IMHA (n=37); anaemic dogs not meeting the inclusion criteria for primary IMHA (n=77) and non‐anaemic dogs (n=113). DNA was extracted from 100 μl of blood and subjected to real‐time quantitative polymerase chain reaction (qPCR) assays for both species of Mycoplasma. Each assay incorporated co‐amplification of canine glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) as an endogenous internal control.R esults : Canine GAPDH was successfully amplified by qPCR from all 227 canine blood samples but none contained M. haemocanis or “ Candidatus M. haematoparvum ” DNA.C linical S ignificance : Haemoplasma infection is uncommon in dogs in the UK and no evidence was found that these organisms act as triggers for IMHA.