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Anaplasma phagocytophilum infection in a dog: identifying the causative agent using PCR
Author(s) -
Kirtz G.,
Meli M.,
Leidinger E.,
Ludwig P.,
Thum D.,
Czettel B.,
Kölbl S.,
Lutz H.
Publication year - 2005
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/j.1748-5827.2005.tb00325.x
Subject(s) - anaplasma phagocytophilum , doxycycline , anaplasma , medicine , 16s ribosomal rna , anaplasmosis , virology , microbiology and biotechnology , polymerase chain reaction , immunofluorescence , gene , biology , immunology , antibody , tick , antibiotics , genetics , borrelia burgdorferi
A diagnosis of Anaplasma phagocytophilum infection was confirmed in a two‐year‐old male golden retriever displaying few clinical and haematological abnormalities. This was achieved by demonstrating ehrlichial organisms in circulating neutrophils, by indirect immunofluorescence assay using A phagocytophilum as an antigen, and by detecting DNA specific for the 16S rRNA gene of granulocytic Anaplasma by PCR. After treatment with doxycycline for 10 days the dog showed improvement and the laboratory values returned to normal.