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Using direct immunofluorescence to detect coronaviruses in peritoneal in peritoneal and pleural effusions
Author(s) -
Parodi M. Cammarata,
Cammarata G.,
Paltrinieri S.,
Lavazza A.,
Ape F.
Publication year - 1993
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/j.1748-5827.1993.tb02591.x
Subject(s) - medicine , feline infectious peritonitis , immunofluorescence , direct fluorescent antibody , cats , ascites , pathology , polyclonal antibodies , indirect immunofluorescence , coronavirus , peritoneum , peritoneal effusion , peritonitis , antiserum , pleural effusion , virology , covid-19 , antigen , gastroenterology , radiology , immunology , antibody , effusion , surgery , disease , infectious disease (medical specialty)
Twenty‐one cases of feline infectious peritonitis (FIP) were diagnosed using a direct immunofluorescence test on cytocentrifuged pleural and peritoneal effusions from cats sampled in vivo (11 cases) and at necropsy (10 cases). A commercial fluorescent polyclonal antiserum of feline origin reacting with FIPV and cross reacting with transmissible gastroenteritis virus and canine coronavirus was used. Eleven cats with ascites of a different origin were used as negative controls. The direct immunofluorescence test was 97 per cent reliable (31 cases of 32) and can be used in routine diagnosis.