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Ultrastructural lesions to the canine intestinal epithelium caused by enteropathogenic E coli
Author(s) -
Hart C. A.,
Embaye H.,
Getty B.,
Saunders J. R.,
Batt R. M.
Publication year - 1990
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/j.1748-5827.1990.tb00699.x
Subject(s) - microbiology and biotechnology , brush border , jejunum , enteropathogenic escherichia coli , ileum , medicine , mucous membrane , pathology , intestinal mucosa , epithelium , microbiological culture , incubation , bacteria , escherichia coli , biology , membrane , biochemistry , vesicle , genetics , gene
Mucosal biopsies from canine jejunum and ileum were established in organ culture, and remained structurally intact for up to 72 hours. Infection of such mucosal biopsies with an enteropathogenic Escherichia coli (EPEC) 0111 results in the characteristic loss of brush border observed during natural and induced infection in other animal species. Within four hours of infection, bacteria had become attached to the mucous overlying the microvilli and by eight hours had penetrated between the microvilli resulting in partial loss of the brush border. After 24 hours incubation there was almost complete effacement of the brush border with cup and pedestal formation at sites of bacterial adherence. We conclude that EPEC should be considered as a potential cause of diarrhoea in the dog and that organ culture of canine mucosal biopsies provides a useful model for studying infection with EPECs and other enteric pathogens.