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The measurement of canine steroid‐induced alkaline phosphatase by L‐phenylalanine inhibition
Author(s) -
ECKERSALL P. D.,
NASH A. S.,
MARSHALL G. M.,
DOUGLAS T. A.
Publication year - 1986
Publication title -
journal of small animal practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 67
eISSN - 1748-5827
pISSN - 0022-4510
DOI - 10.1111/j.1748-5827.1986.tb02155.x
Subject(s) - alkaline phosphatase , isozyme , steroid , phenylalanine , medicine , phosphatase , endocrinology , enzyme , biochemistry , biology , amino acid , hormone
Canine steroid‐induced alkaline phosphatase (SIAP) has been measured quantitatively in an assay which depends on the finding that L‐phenylalanine has a greater inhibitory effect on SIAP than on other plasma isoenzymes. The assay has advantages in precision, practicability and availability over other assays for SIAP. The steroid‐induced isoenzyme was shown to contribute over 40 per cent of the total alkaline phosphatase (AP) activity in over 60 per cent of cases in which the level of AP was raised.